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PhD Seminar - Haoyu Xiong

University of Otago

Ice activities in New Zealand Chionochloa species

Biochemistry Seminar Room 231
Jul. 11, 2017

Chionochloa are tussocks mainly endemic to New Zealand and dominate the alpine grasslands. Seasonal variations of ice activities were found in most Chionochloa collections (13 of 17) with winter collections showed the highest activities. Intrinsic proteins were essential in maintaining all three types of ice activities, which showed different responses to various treatments. Ice affinity purification indicated the presence of ice active proteins (IAP) in winter C. macra, which was confirmed by mass spectrometry, transcriptome analysis, and protein expression in E. coli. Phylogeny analysis of IAP genes and cold related genes indicated these genes from C. macra did not split with the core Pooideae family.