Professor Julian Eaton-Rye

JulianEaton Rye
  • Biochemistry Department
  • School of Biomedical Sciences
  • University of Otago
  • P.O. Box 56
  • 710 Cumberland St
  • Dunedin 9054 , New Zealand
  • Tel.: 64 3 479-7865
  • Fax: 64 3 479-7866
  • Email:julian.eaton-rye@otago.ac.nz

Life on our planet is driven by Photosystem II that captures sunlight and extracts electrons from H2O to reduce CO2 into sugars while releasing the O2 we breathe. My laboratory concentrates on structure-function studies of Photosystem II in plants and cyanobacteria.

Currently we are working on protein-protein interactions that facilitate sustained water-splitting activity by Photosystem II in response to changing environmental conditions such as fluctuating light levels, temperature and pH. We are also interested in protein factors that are required for the assembly of Photosystem II.

Since the sun provides more energy in 1 h than our entire global energy consumption in 1 year, we are also working on metabolic engineering to couple the harvesting of light by photosynthetic protein complexes to the efficient production of biofuels in cyanobacteria. 

Media appearances

Professor Eaton-Rye on "Our Changing World" in February 2015:

"Unlocking the Secrets of Photosynthesis" at Radio NZ National

Professor Eaton-Rye was featured in two articles in the New Zealand Herald in October 2014:

"Can Universities save the planet?" at the New Zealand Herald

"Five ideas to improve the world" at the New Zealand Herald

Professor Eaton-Rye was interviewed on Channel 9 in January 2014

See the video on Channel 9's site

Professor Julian Eaton-Rye's Inaugural Professorial Lecture

Professor Julian Eaton-Rye was on Australia's Radio National in May 2012

Go to the RadioNational page to hear the audio

Members of Professor Julian Eaton-Rye's laboratory were featured on the TVNZ 7 programme "Ever Wondered" in early August 2011

Watch the video at TVNZ On Demand (go to chapter 3)

Recent Publications

Crawford, T.S., Hanning, K.R., Chua, J.P.S., Eaton-Rye, J.J. and Summerfield, T.C. Comparison of D1′- and D1-containing PS II reaction centre complexes under different environmental conditions in Synechocystis sp. PCC 6803. Plant Cell & Environment, in press (2016)

Najafpour, M.M., Renger, G., Holynska, M., Moghaddam, A.N., Aro, E.-M., Carpentier, R., Nishihara, H., Eaton-Rye, J.J., Shen, J.-R. and Allakhverdiev, S.I. Manganese compounds as water-oxidizing catalysts: From the natural water-oxidizing complex to nanosized manganese oxide structures. Chemical Reviews 116: 2886-2936 (2016)

Jackson, S.A., Eaton-Rye, J.J., Bryant, D.A., Posewitz, M.C. and Davies, F.K. Dynamics of photosynthesis in a glycogen-deficient glgC mutant of Synechococcus sp. strain PCC 7002. Applied and Environmental Microbiology 81: 6210-6222 (2015)

Nath, K., Najafpour, M.M., Voloshin, R.A., Balaghi, S.E., Tyystjarvi, E., Timilsina, R., Eaton-Rye, J.J. et al. Photobiological hydrogen production and artificial photosynthesis for clean energy: from bio to nanotechnologies. Photosynthesis Research 126: 237-247 (2015)

Jackson, S.A. and Eaton-Rye, J.J. Characterization of a Synechocystis sp. PCC 6803 double mutant lacking the CyanoP and Ycf48 proteins of Photosystem II. Photosynthesis Research 124: 217-229 (2015)

Najafpour, M.M., Fekete, M., Sedigh, D.J., Aro, E.-M., Carpentier, R., Eaton-Rye, J.J., Nishihara, H., Shen, J.-R., Allakhverdiev, S.I. and Spiccia, L. Damage management in water-oxidizing catalysts: From Photosystem II to nanosized metal oxides. Catalysis 5: 1499-1512 (2015)

Jackson, S.A., Hervey, J.R.D., Dale, A.J. and Eaton-Rye, J.J. Removal of both Ycf48 and Psb27 in Synechocystis sp. PCC 6803 disrupts Photosystem II assembly and alters QA- oxidation in the mature complex. FEBS Letters 588: 3751-3760 (2014)

Lamb, J.J., Hill, R.E., Eaton-Rye, J.J. and Hohmann-Marriott, M.F. Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803. PLoS ONE 9: e105761. doi:10.1371/journal.pone.0105761 (2014)

Mabbitt, P.D., Wilbanks, S.M. and Eaton-Rye, J.J. Structure and function of the hydrophilic Photosystem II assembly proteins: Psb27, Psb28 and Ycf48. Plant Physiology and Biochemistry 81: 96-107 (2014)

Luo, H., Jackson, S.A., Fagerlund, R.D., Summerfield, T.C. and Eaton-Rye, J.J. The importance of the hydrophilic region of PsbL for the plastoquinone electron acceptor complex of Photosystem II. Biochimica et Biophysica Acta 1837: 1435-1446 (2014)

Najafpour, M.M., Isaloo, M.A., Eaton-Rye, J.J., Tomo, T., Nishihara, H., Satoh, K., Carpentier, R., Shen, J.-R. and Allakhverdiev, S.I. Water exchange rate in manganese-based water-oxidizing catalyts in photosynthetic systems: From the water-oxidizing complex in Photosystem II to nano-sized manganese oxides. Biochimica et Biophysica Acta 1837: 1395-1410 (2014)

Hill, R.E. and Eaton-Rye, J.J. Plasmid construction by SLIC or sequence and ligation-independent cloning. In DNA Cloning and Assembly Methods. S. Valla and R. Lale, eds., Methods in Molecular Biology 1116. Humana Press, Springer, New York, pp 25-36 (2014)

Najafpour, M.M., Ghobadi, M.Z., Haghighi, B., Eaton-Rye, J.J., Tomo, T., Shen, J.-R. and Allakhverdiev, S.I. Nano-sized manganese-—calcium cluster in Photosystem II. Biochemistry (Moscow) 79: 324-336 (2014)

Najafpour, M.M., Sedigh, D.J., Eaton-Rye, J.J. and Allakhverdiev, S.I. The water-oxidizing complex in Photosystem II. In Contemporary Problems of Photosynthesis, S.I. Allakhverdiev, A.B. Rubin and V.A. Shuvalov, eds. Institute of Computer Science, Izhevsk-Moscow, Vol. 1, pp. 325-341 (2014)

Mabbitt, P.D., Wilbanks, S.W. and Eaton-Rye, J.J. Duplication and divergence of the Psb27 subunit of Photosystem II in the green algal lineage. New Zealand Journal of Botany 52: 36-47 (2014)

Morris, J.N., Crawford, T.S., Jeffs, A., Stockwell, P.A., Eaton-Rye, J.J. and Summerfield, T.C. Whole genome re-sequencing of two 'wild-type' strains of the model cyanobacterium Synechocystis sp. PCC 6803. New Zealand Journal of Botany 52: 74-83 (2014)

Eaton-Rye, J.J., Packer, M.A., Summerfield, T.C. and Wood, S.A. eds. Algal and cyanobacterial bioenergy and diversity. New Zealand Journal of Botany 52: 1-159 (2014)

Mabbitt, P.D., Eaton-Rye, J.J. and Wilbanks, S.M. Mutational analysis of the stability of Psb27 from Synechocystis sp. PCC 6803: Implications for models of Psb27 structure and binding to CP43. European Biophysics Journal 42: 787-793 (2013)

Najafpour, M.M., Rahimi, F., Sedigh, D.J., Carpentier, R., Eaton-Rye, J.J., Shen, J.-R. and Allakhverdiev, S.I. Gold or silver deposited on layered manganese oxide: a functional model for the water-oxidizing complex of Photosystem II. Photosynthesis Research 117: 423-429 (2013)

Najafpour, M.M., Tabrizi, M.A., Haghighi, B., Eaton-Rye, J.J., Carpentier, R. and Allakhverdiev, S.I. Imidazolium or guanidinium/layered manganese (III, IV) oxide hybrid as a promising structural model for the water-oxidizing complex of Photosystem II for artificial photosynthetic systems. Photosynthesis Research 117: 413-421 (2013)

Eaton-Rye, J.J. Govindjee at 80: more than 50 years of free energy for photosynthesis. Photosynthesis Research 116: 111-144 (2013)

Summerfield, T.C., Crawford, T.S., Young, R.D., Chua, J.P.S., Macdonald, R.L., Sherman, L.A. and Eaton-Rye, J.J. Environmental pH affects photoautotrophic growth of Synechocystis sp. PCC 6803 strains carrying mutations in the lumenal proteins of Photosystem II. Plant & Cell Physiology 54: 859-874 (2013)

Lamb, J.J., Eaton-Rye, J.J. and Hohmann-Marriott, M.F. A cost effective solution to reliable determination of cell numbers of microorganisms in liquid culture. Current Microbiology 67: 123-129 (2013)

Burut-Archanai, S., Eaton-Rye, J.J., Incharoensakdi, A. and Powtongsook, S. Phosphorus removal in a closed recirculating aquaculture system using the cyanobacterium Synechocystis sp. PCC 6803 strain lacking the SphU regulator of the Pho regulon. Biochemical Engineering Journal 74: 69-75 (2013)

Jackson, S.A., Hinds, M.G. and Eaton-Rye, J.J. Solution structure of CyanoP from Synechocystis sp. PCC 6803: New insights on the structural basis for functional specialization amongst PsbP family proteins. Biochimica et Biophysica Acta 1817: 1331-1338 (2012)

Shevela, D., Eaton-Rye, J.J., Shen, J.-R. and Govindjee. Photosystem II and the unique role of bicarbonate: A historical perspective. Biochimica et Biophysica Acta 1817: 1134-1151 (2012)

Najafpour, M.M., Moghaddam, A.N., Yang, Y.N., Aro, E.-M., Carpentier, R., Eaton-Rye, J.J., Lee, C.-H. and Allakhverdiev, S.I. Biological water-oxidizing complex: A nano-sized manganese-calcium oxide in a protein environment. Photosynthesis Research 114: 1-13 (2012)

Lamb, J.J., Eaton-Rye, J.J. and Hohmann-Marriott, M.F. An LED-based fluorometer for chlorophyll quantification in the laboratory and in the field. Photosynthesis Research 114: 59-68 (2012)

Bricker, T.M., Roose, J.L., Fagerlund, R.D., Frankel, L.K. and Eaton-Rye, J.J. The extrinsic proteins of Photosystem II. Biochimica et Biophysica Acta 1817: 121-142 (2012)

Vos, M.H., Bouzhir-Sima, L., Lambry, J.-C., Luo, H., Eaton-Rye, J.J., Ioanoviciu, A., Ortiz de Montellano, P.R. and Liebl, U. Ultrafast ligand dynamics in the heme-based GAF sensor domains of the histidine kinases DosS and DosT from Mycobacterium tuberculosis. Biochemistry 51: 159-166 (2012)

Eaton-Rye, J.J., Tripathy, B.C. and Sharkey, T.D. eds. Photosynthesis: Plastid Biology, Energy Conversion and Carbon AssimilationAdvances in Photosynthesis and Respiration 34. Springer, Dordrecht, 892 pages (2012)

McConnell, I.L., Eaton-Rye, J.J. and Van Rensen, J.J.S. Regulation of Photosystem II electron transport by bicarbonate. In Photosynthesis: Plastid Biology, Energy Conversion and Carbon Assimilation, J.J. Eaton-Rye, B.C. Tripathy and T.D. Sharkey, eds. Advances in Photosynthesis and Respiration 34. Springer, Dordrecht, pp 475-500 (2012)

Burut-Archanai, S., Eaton-Rye, J.J. and Incharoensakdi, A. Na+-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter. BMC Microbiology 11: 225 (2011)

Ehimen, E.A., Sun, Z.F., Carrington, C.G., Birch, E.J. and Eaton-Rye, J.J. Anaerobic digestion of microalgae residues resulting from the biodiesel production process. Applied Energy 88: 3454-3463 (2011)

Fagerlund, R.D. and Eaton-Rye, J.J. The lipoproteins of cyanobacterial Photosystem II. Journal of Photochemistry and Photobiology B: Biology 104: 191-203 (2011)

Nagarajan, A., Winter, R., Eaton-Rye, J. and Burnap, R. A synthetic DNA and fusion PCR approach to the ectopic expression of high levels of the D1 protein of Photosystem II in Synechocystis sp. PCC 6803. Journal of Photochemistry and Photobiology B: Biology 104: 212-219 (2011)

Eaton-Rye, J.J. Construction of gene interruptions and gene deletions in the cyanobacterium Synechocystis sp. strain PCC 6803. In Photosynthesis Research Protocols, 2nd Edition. R. Carpentier, ed., Methods in Molecular Biology 684. Humana Press, Springer, New York, pp 295-312 (2011)

Jackson, S.A., Fagerlund, R.D., Wilbanks, S.M. and Eaton-Rye, J.J. Crystal structure of PsbQ from Synechocystis sp. PCC 6803 at 1.8 Å: Implications for binding and function in cyanobacterial Photosystem II. Biochemistry 49: 2765–2767 (2010)

Hohmann-Marriott, M.F., Takizawa, K., Eaton-Rye, J.J., Mets, L. and Minagawa, J. The redox state of the plastoquinone pool directly modulates minimum chlorophyll fluorescence yield in Chlamydomonas reinhardtiiFEBS Letters 584: 1021–1026 (2010)

Mabbitt, P.D., Rautureau, G.J.P., Day, C.L., Wilbanks, S.M., Eaton-Rye, J.J. and Hinds, M.G. Solution structure of Psb27 from cyanobacterial Photosystem II. Biochemistry 48: 8771-8773 (2009)

Burut-Archanai, S., Incharoensakdi, A. and Eaton-Rye, J.J. The extended N-terminal region of SphS is required for detection of external phosphate levels in Synechocystis sp. PCC 6803. Biochemical and Biophysical Research Communications 378: 383-388 (2009)

Luo, H. and Eaton-Rye, J.J. Directed mutagenesis of the transmembrane domain of the PsbL subunit of Photosystem II in Synechocystis sp. PCC 6803. Photosynthesis Research 98: 337-347 (2008)

Bentley, F.K., Luo, H., Dilbeck, P., Burnap, R.L. and Eaton-Rye, J.J. Effects of inactivating psbM and psbT on photodamage and assembly of Photosystem II in Synechocystis sp. PCC 6803. Biochemistry 47: 11637-11646 (2008)

Eaton-Rye, J.J. ed. Govindjee special issue: Part B, Celebrating Govindjee's 50 years in photosynthesis research and his 75th birthday. Photosynthesis Research 94: 153-466 (2007)

Eaton-Rye, J.J. ed. Govindjee special issue: Part A, Celebrating Govindjee's 50 years in photosynthesis research and his 75th birthday. Photosynthesis Research 93: 1-244 (2007)

Juntarajumnong, W., Hirani, T.A., Simpson, J.M., Incharoensakdi, A. and Eaton-Rye, J.J. Phosphate sensing in Synechocystis sp. PCC 6803: SphU and the SphS-SphR two-component regulatory system. Archives of Microbiology 188: 389-402 (2007)

Summerfield, T.C., Eaton-Rye, J.J. and Sherman, L.A. Global gene expression of a ?PsbO:?PsbU mutant and a spontaneous revertant in the cyanobacterium Synechocystis sp. strain PCC 6803. Photosynthesis Research 94: 265-274 (2007)

Juntarajumnong, W., Eaton-Rye, J.J. and Incharoensakdi, A. Two-component signal transduction in Synechocystis sp. PCC 6803 under phosphate limitation: Role of acetyl phosphate. Journal of Biochemistry and Molecular Biology 40: 708-714 (2007)

Juntarajumnong, W., Incharoensakdi, A. and Eaton-Rye, J.J. Identification of the start codon for sphS encoding the phosphate-sensing histidine kinase in Synechocystis sp. PCC 6803. Current Microbiology 55: 142-146 (2007)

PhD projects available in this laboratory

Selected Publications

Tim S Crawford, Kyrin R Hanning, Jocelyn P S Chua, Julian J Eaton-Rye, and Tina C Summerfield., Comparison of D1´- and D1-Containing PS II Reaction Centre Complexes Under Different Environmental Conditions in Synechocystis sp. PCC 6803., Plant Cell Environ 2016, Link »

Mohammad Mahdi Najafpour, Gernot Renger, Małgorzata Hołyńska, Atefeh Nemati Moghaddam, Eva-Mari Aro, Robert Carpentier, Hiroshi Nishihara, Julian J Eaton-Rye, Jian-Ren Shen, and Suleyman I Allakhverdiev., Manganese Compounds as Water-Oxidizing Catalysts: From the Natural Water-Oxidizing Complex to Nanosized Manganese Oxide Structures., Chem Rev 2016 p. acs.chemrev.5b00340., Link »