Associate Professor Alan Carne

Senior Lecturer
AlanCarne3
  • Biochemistry Department
  • School of Biomedical Sciences
  • University of Otago
  • P.O. Box 56
  • 710 Cumberland St
  • Dunedin 9054 , New Zealand
  • Tel.: 64 3 479-7855
  • Fax: 64 3 479-7866
  • Email:alan.carne@otago.ac.nz

Research interests:

My laboratory is involved with various protein-based research projects.

  1. Utilisation of natural product protein-containing materials that are by-products or waste products of processing, such as milk cheese whey, meat and blood waste, oilseed cake residue
  2. Production of bioactive peptides from protein-containing by-product/waste product materials, using proteases of non-gut origin
  3. Characterisation of milk protein fractions from different species
  4.  Meat tenderising
  5. Characterisation of carotenoid and polyhydroxynaphthoquinone pigments from sea urchin and their binding interactions with proteins.
  6. Encapsulation of health-promoting molecules.
  7. Several other protein-based collaborative research projects.

We use a variety of protein purification and analysis methods, including 1D- and 2D-electrophoresis (both mini-gel and large format), OFFGEL isoelectric focussing, western blotting, FPLC and HPLC of proteins, peptides and pigments, use of mass spectrometry workflows for proteomic analysis, and various biophysical analysis methods in collaboration with other researchers. Protease assays, bioactive peptide in vitro bioassays and more specialised cell-based assays.

Coomassie blue stained 1D-PAGE of protein fractions

;

Silver stained large format 2D-PAGE protein extract display

Ion exchange chromatogram of fractionated proteins

Awards

  • Daniel Garama
  • 2012, Te Amorangi National Maori Excellence Award for doctoral studies

Selected Publications

Mina Tirgar, Patrick Silcock, Alan Carne, and E John Birch., Effect of extraction method on functional properties of flaxseed protein concentrates., Food Chemistry 2017 vol. 215 pp. 417-424., Link »

A Shavandi, A.E.-D.A. Bekhit, A Carne, and A Bekhit., Evaluation of keratin extraction from wool by chemical methods for bio-polymer application., Journal of Bioactive and Compatible Polymers 2016., Link »

Jodi Pilbrow, Alaa El-Din A Bekhit, and Alan Carne., Fractionation of sheep cheese whey by a scalable method to sequentially isolate bioactive proteins., Food Chemistry 2016 vol. 203 pp. 165-174., Link »

Sue-Siang Teh, Alaa El-Din A Bekhit, Alan Carne, and John Birch., Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain., Food Chemistry 2016 vol. 203 pp. 199-206., Link »

Clara S F Bah, Alan Carne, Michelle A McConnell, Sonya Mros, and Alaa El-Din A Bekhit., Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations., Food Chemistry 2016 vol. 202 pp. 458-466., Link »

Clara S F Bah, Alaa El-Din A Bekhit, Michelle A McConnell, and Alan Carne., Generation of bioactive peptide hydrolysates from cattle plasma using plant and fungal proteases., Food Chemistry 2016 vol. 213 pp. 98-107., Link »

Yakun Hou, Amin Shavandi, Alan Carne, Adnan A Bekhit, Tzi Bun Ng, Randy Chi Fai Cheung, and Alaa El-Din A Bekhit., Marine shells: Potential opportunities for extraction of functional and health-promoting materials., Critical Reviews in Environmental Science and Technology 2016 vol. 46 (11-12) pp. 1047-1116., Link »

Kate Ryder, Alaa El-Din Bekhit, Michelle McConnell, and Alan Carne., Towards generation of bioactive peptides from meat industry waste proteins: Generation of peptides using commercial microbial proteases., Food Chemistry 2016 vol. 208 pp. 42-50., Link »

Minh Ha, Manya Sabherwal, Elizabeth Duncan, Stewart Stevens, Peter Stockwell, Michelle McConnell, Alaa El-Din Bekhit, and Alan Carne., In-Depth Characterization of Sheep (Ovis aries) Milk Whey Proteome and Comparison with Cow (Bos taurus)., PLoS ONE 2015 vol. 10 (10) p. e0139774., Link »

S S Teh, B E Niven, A.E.-D.A. Bekhit, A Carne, and J Birch., Optimization of polyphenol extraction and antioxidant activities of extracts from defatted flax seed cake (Linum usitatissimum L.) using microwave-assisted and pulsed electric field (PEF) technologies with response surface methodology., Food Science and Biotechnology 2015 vol. 24 (5) pp. 1649-1659., Link »

Alaa El-Din A Bekhit, Via Suwandy, Alan Carne, Remy van de Ven, and David L Hopkins., Effect of repeated pulsed electric field treatment on the quality of hot-boned beef loins and topsides., Meat Science 2015 vol. 111 pp. 139-146., Link »

Bah, C. S. F., Bekhit, A. E.-D. A., Carne, A., & Mcconnell, M. A., Production of bioactive peptide hydrolysates from deer, sheep and pig plasma using plant and fungal protease preparations. , Food Chemistry (2015) 176, 54–63., Link »

S S Teh, B E Niven, A.E.-D.A. Bekhit, A Carne, and E. J. Birch., Microwave and pulsed electric field assisted extractions of polyphenols from defatted canola seed cake., Int J Food Sci Technol 2015 vol. 50 (5) pp. 1109-1115., Link »

Ryder, K., Ha, M., Bekhit, A. E.-D., & Carne, A., Characterisation of novel fungal and bacterial protease preparations and evaluation of their ability to hydrolyse meat myofibrillar and connective tissue proteins., Food Chemistry (2015) 172C, 197–206., Link »

V Suwandy, A Carne, R van de Ven, A.E.-D.A. Bekhit, and D.L. Hopkins., Effect of Pulsed Electric Field Treatment on the Eating and Keeping Qualities of Cold-Boned Beef Loins: Impact of Initial pH and Fibre Orientation., Food and Bioprocess Technology 2015, Link »

Via Suwandy, Alan Carne, Remy van de Ven, Alaa El-Din A Bekhit, and David L Hopkins., Effect of pulsed electric field treatment on hot-boned muscles of different potential tenderness., Meat Science 2013 pp. -, Link »

V Suwandy, A Carne, R van de Ven, A.E.-D.A. Bekhit, and D.L. Hopkins., Effect of Repeated Pulsed Electric Field Treatment on the Quality of Cold-Boned Beef Loins and Topsides., Food and Bioprocess Technology 2015, Link »

Suwandy, V., Carne, A., van de Ven, R., Bekhit, A. E.-D. A., & Hopkins, D. L. , Effect of pulsed electric field on the proteolysis of cold boned beef M: Longissimus lumborum and M: Semimembranosus., Meat Science (2015). 100, 222–226., Link »

Bah, C. S., Bekhit, A. E.-D. A., Carne, A., & Mcconnell, M. A, Composition and biological activities of slaughterhouse blood from red deer, sheep, pig and cattle., Journal of the Science of Food and Agriculture. (2015)., Link »

Sue-Siang Teh, Brian E Niven, Alaa El-Din A Bekhit, Alan Carne, and Edward John Birch., Microwave and pulsed electric field assisted extractions of polyphenols from defatted canola seed cake., Int J Food Sci Techno 2015 , Link »

Jodi Pilbrow, Manya Sabherwal, Daniel Garama, and Alan Carne., A novel fatty acid-binding protein-like carotenoid-binding protein from the gonad of the New Zealand sea urchin Evechinus chloroticus., PLoS ONE 2013 vol. 9 (9) pp. , Link »

Johnson, S. L., Villarroel, M., Rosengrave, P., Carne, A., Kleffmann, T., Lokman, P. M., & Gemmell, N. J. , Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid., PLoS ONE. 9(8), e104155 (2014)., Link »

M. Ha, A E D Bekhit, M McConnell, S Mason, and A Carne, Fractionation of whey proteins from red deer (Cervus elaphus) milk and comparison with whey proteins from cow, sheep and goat milks, Small Ruminant Research 2014 vol. 120 (1) pp. 125-134, Link »

S S Teh, A E D Bekhit, A Carne, and J Birch, Effect of the defatting process, acid and alkali extraction on the physicochemical and functional properties of hemp, flax and canola seed cake protein isolates, Journal of Food Measurement and Characterization 2014 vol. 8 (2) pp. 92-104, Link »

S S Teh, B E Niven, A.E.-D.A. Bekhit, A Carne, and E. J. Birch, The Use of Microwave and Pulsed Electric Field as a Pretreatment Step in Ultrasonic Extraction of Polyphenols from Defatted Hemp Seed Cake (Cannabis sativa) Using Response Surface Methodology, Food and Bioprocess Technology 2014, Link »

Minh Ha, Alaa El-Din Bekhit, and Alan Carne, Effects of l- and iso-ascorbic acid on meat protein hydrolyzing activity of four commercial plant and three microbial protease preparations., Food Chemistry 2014 vol. 149 pp. 1-9, Link »

S Gebhard, C Fang, A Shaaly, D J Leslie, M R Weimar, F. Kalamorz, A Carne, and G M Cook, Identification and characterization of a bacitracin resistance network in Enterococcus faecalis, Antimicrobial Agents and Chemotherapy 2014 vol. 58 (3) pp. 1425-1433, Link »

Alaa El-Din A Bekhit, Alan Carne, Minh Ha, and Philip Franks, Physical Interventions to Manipulate Texture and Tenderness of Fresh Meat: a Review, International Journal of Food Properties 2014 vol. 17 (2) pp. 433-453, Link »

C S F Bah, A.E.-D.A. Bekhit, A Carne, and M A Mcconnell, Slaughterhouse blood: An emerging source of bioactive compounds, Comprehensive Reviews in Food Science and Food Safety 2013 vol. 12 (3) pp. 314-331, Link »

Minh Ha, Alaa El-Din Bekhit, Alan Carne, and David L Hopkins, Comparison of the Proteolytic Activities of New Commercially Available Bacterial and Fungal Proteases toward Meat Proteins., Journal of food science , Link »

Minh Ha, Alaa El-Din Bekhit, Alan Carne, and David L Hopkins, Characterisation of kiwifruit and asparagus enzyme extracts, and their activities toward meat proteins., Food Chemistry 2013 vol. 136 (2) pp. 989-998, Link »

A E D Bekhit, D.L. Hopkins, M M Farouk, and A Carne., Interventions to improve the tenderness of fresh meat: A future prospect for camel meat research., Camel Meat and Meat Products. 2013 Book Chapter,

M. Ha, A.E.-D.A. Bekhit, A Carne, and D.L. Hopkins, Characterisation of commercial papain, bromelain, actinidin and zingibain protease preparations and their activities toward meat proteins, Food Chemistry 2012 vol. 134 (1) pp. 95-105, Link »

Daniel Garama, Phil Bremer, and Alan Carne, Extraction and analysis of carotenoids from the New Zealand sea urchin Evechinus chloroticus gonads., Acta biochimica Polonica 2012 vol. 59 (1) pp. 83-85, Link »

Jodi Pilbrow, Daniel Garama, and Alan Carne, Carotenoid-binding proteins; accessories to carotenoid function., Acta biochimica Polonica 2012 vol. 59 (1) pp. 163-165,

Euan J Rodger, Rachel J Suetani, Gregory T Jones, Torsten Kleffmann, Alan Carne, Michael Legge, and Sally P A McCormick, Proteomic analysis of aortae from human lipoprotein(a) transgenic mice shows an early metabolic response independent of atherosclerosis., PLoS ONE 2012 vol. 7 (1) p. e30383, Link »

Zahra Haddadian, Graham T Eyres, Alan Carne, David W Everett, and Phil Bremer., Impact of different milk fat globule membrane preparations on protein composition, xanthine oxidase activity, and redox potential., International Dairy Journal 2017 vol. 64 pp. 14-21., Link »

Ya-Fei Liu, Indrawati Oey, Phil Bremer, Alan Carne, and Pat Silcock., Effects of pH, temperature and pulsed electric fields on the turbidity and protein aggregation of ovomucin-depleted egg white., Food Research International 2017 vol. 91 pp. 161-170., Link »