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Y Nakatani, D S Larsen, S.M. Cutfield, and J.F. Cutfield, Biochemistry, 2014 vol. 53 (20) pp. 3318-3326
By changing just one amino acid in an enzyme found in the cell wall of the pathogenic fungus Candida albicans, we can make it add on a sugar molecule to another molecule instead of its usual action of chopping it off. Using the techniques of mass spectrometry, NMR spectroscopy and X-ray crystallography we have shown how this chemical reaction occurs and revealed some surprising findings on how sugar molecules can attach themselves to the surface of the enzyme.